Identification of ALP+/CD73+ defining markers for enhanced osteogenic potential in human adipose-derived mesenchymal stromal cells by mass cytometry

Abstract Background The impressive progress in the field of stem cell research past decades has provided ground for development cell-based therapy. Mesenchymal stromal cells obtained from adipose tissue (AD-MSCs) represent a viable source therapies. However, heterogeneity and variable differentiation ability AD-MSCs depend on cellular composition strong limitation their use therapeutic applications. In order to fully understand MSC preparations, it would be essential analyze at single-cell level. Method Recent advances technologies have opened way high-dimensional, high-throughput, high-resolution measurements biological systems. We made cytometry by time-of-flight (CyTOF) technology explore 17 human AD-MSCs, interrogating 31 markers Subcellular was investigated naïve state as well during osteogenic commitment, via unsupervised dimensionality reduction supervised representation learning approaches. Result This study showed high variability subcellular upon isolation prolonged culture. Algorithm-guided identification emerging subpopulations allowed an ALP+/CD73+ subpopulation with enhanced potential. could demonstrate vitro that sorted exhibited potential is moreover fundamental lineage commitment. finally this present freshly isolated adipose-derived vascular fractions (SVFs) ultimately used Conclusion data reveal, level, several donors highlight how impacts capacity. marker combination (ALP/CD73) can not only assess undifferentiated AD-MSC but also employed prospectively enrich fraction